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Product Info Summary
| SKU: | LS081556 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of chromosome 15 open reading frame 44 (C15orf44), transcript variant 2 |
Product info
Product Name
C15orf44 (VWA9) (NM_030800) Human Over-expression Lysate
SKU/Catalog Number
LS081556
Size
100 µg
Description
Transient overexpression lysate of chromosome 15 open reading frame 44 (C15orf44), transcript variant 2
Storage & Handling
Cite This Product
C15orf44 (VWA9) (NM_030800) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081556)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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C15orf44 (VWA9) Human Over-expression Lysates NM_030800.
Western blot validation of overexpression lysate (LS081556) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with C15orf44 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for INTS14 (Source: Uniprot.org, NCBI)
Gene Name
INTS14
Full Name
Integrator complex subunit 14
Weight
57.471kDa
Superfamily
INTS14 family
Alternative Names
Integrator complex subunit 14 INTS14 C15orf44, VWA9 integrator complex subunit 14 integrator complex subunit 14|UPF0464 protein C15orf44|von Willebrand factor A domain containing 9|von Willebrand factor A domain-containing protein 9
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on INTS14, check out the INTS14 Infographic
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In this infographic, you will see the following information for INTS14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For C15orf44 (VWA9) (NM_030800) Human Over-expression Lysate (LS081556)
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No publications found for LS081556
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